Breast Nipple SecretionsIndications:
Detection of both benign and malignant lesions of the breast.
Direct smear of nipple discharge.
1. Two glass slides (single-end frosted)
2. Fixative (95%) Alcohol
3. Specimen Requisition
4. Specimen Bag with a biohazard label
1. Using a lead pencil, label the slides with the patient's name and date prior to collection.
2. Collect a small amount of nipple secretion directly onto one of the slides. If the nipple
appears crusty, gently remove crust with saline saturated gauze pads. Using pressure
around areola area and nipple try to express fluid. As a drop appears, touch the glass
slide to the nipple using a touch-roll motion.
3. Oppose a second glass slide onto the first, and then gently and quickly pull the two
slides apart to distribute the material in a thin film over both slides.
4. Fix the smears immediately by immersing into a container of 95% alcohol. Alternatively,
slides may be allowed to completely air dry.
5. Submit the specimen and completed requisition to CPA.
Complete test requisition including last and first name of patient, patient's date of birth and social security number, body site and source of specimen collected. Label specimen container (using the labels provided on the requisition) with patient's first name and last name, and body site/source. The container must have at least two (2) unique identifiers. Examples of unique identifiers: patient name, DOB, unique bar code, etc. Include pertinent clinical information, i.e., previous malignancy, radiation therapy, drugs, etc. Place container in a specimen bag with a biohazard label. Place the requisition in the side pocket of the specimen bag.
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